Quench–Gone Organic Modified (QGO–M) is based on the measurement of ATP and is designed for organic–based samples, such as metalworking emulsions or fuels.

QGO–M includes special provisions to separate organics prior to analysis, allowing you to quickly measure total microbiological concentration using this kit with a wide detection range and no assay interference! Use QGO–M to detect total microbiological activity in:

For filterable chemical products including personal care products, polymers, and admixtures, use Quench-Gone Organic Modified, High-Sensitivity/Pre-Dilution (QGOM-XLPD).

Quench–Gone technology from LuminUltra™ is based on the measurement of Adenosine Triphosphate (ATP). ATP is a direct and interference–free indicator of total living biomass. ATP is measured using the firefly luciferase assay, where a sample containing ATP is introduced to a solution containing the enzyme Luciferase, which naturally occurs in the tails of fire flies, to produce light. The light is detected in a luminometer.

QGO–M uses one analysis of Cellular ATP (cATP™) to directly determine the total microbiological concentration. QGO–M test kits use filtration to concentrate the biomass contained in water sample and separate out organic compounds, thereby removing inhibition and increasing sensitivity. QGO–M is optimized to detect between 1 and 200,000pg ATP/mL using standard procedures and equipment, depending on the type or volume of sample analyzed.

* There is no limit to QGO–M sensitivity. Procedural adaptations can be used to amplify the low–range sensitivity and to accommodate streams with large amounts of solids – these adaptations require additional equipment (LumiFilter™).

How to Get Started

LuminUltra's test kits contain all of the consumable materials required to run their specified number of tests (Defined by the last 2 or 3 digits of the product code). To use these test kits, LuminUltra recommends one of the following equipment bundles:

• Field Kit & Luminometer (EQP-PAC): Field Case, Micropipettors, Kikkoman Lumitester.
• Bench Materials & Luminometer (EQP-BTM): Micropipettors, Kikkoman Lumitester, Test Tube Racks.
• Kikkoman Lumitester TM C-110 (EQP-LUK): Kikkoman Lumitester luminometer & accessories.

NOTE: LuminUltra’s test kits can be used with the majority of photomultiplier tube–based luminometers. Contact LuminUltra to confirm compatibility of your luminometer.

LuminUltra is sensitive to the needs of each individual customer.  Our expert staff can consult with you to determine the best means by which the data collected from our test kits can be integrated into your existing data management practices.  We have multiple software platforms and can even assist you to integrate ATP data directly into existing control systems, process historians, and data archives.

Getting started with QGO-M is easier with direct training.  LuminUltra can supply you with on-site auditing and training services, web-based training, and one-on-one consultation to get your process improvement program off the ground.  Contact us today to learn more!

Download detailed Test Kit Instructions	View Demo Video

Test Kit Contents and Storage

When you receive your QGO-M test kit, utilize the following guidelines for material storage.  Note that the presence and quantity of each item listed below will depend on test kit size and type.

* Luminase shelf life can be extended to 6 months when frozen, or can be left at room temperature for as long as 3 weeks during routine use.  Note that the Luminase supplied in QGO-M kits is NOT interchangeable with other forms of Luminase (i.e.  Luminase^W, Luminase Lite, and Luminase^XL).
** LumiClean is a solvent and has flammable properties.  Store below 55oC at all times to prevent boiling.  Consult MSDS sheet for more information and be sure to follow storage and handling guidelines.
*** Note that Quench-Gone Organic filters are optimized for compatibility with all organic fluids, including biofuels.  These filters are recommended for use in all QGO-M analyses, although regular Quench-Gone filters may be used to test less aggressive fluids such as oil-contaminated water.
**** Pipette tips supplied in complete test kits are compatible with most Fisherbrand and Eppendorf adjustable micropipettors. 
NOTE: If your application requires greater sensitivity, inquire about our XL kit option.