Documents
QuenchGone21 Industrial (QG21I)
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Performing Tests - QuenchGone21 IndustrialQG21I analyses require two parallel tests for each sample:
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1. Total ATP (tATP) The Total ATP test detects all ATP contained in the sample, inside and outside the cells. This is achieved by lysing the living cells and measuring their ATP as well as ATP that has already been released from dead cells. |
tATP Analyses consist of three steps:    |
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2. Dissolved ATP (dATP) The Dissolved ATP test uses the specialized LumiSolve dilution buffer to stabilize extracellular ATP (from dead and stressed cells) and also makes bound, or “complexed” ATP soluble. This allows ATP from outside of living cells to be measured without harming living cells. |
dATP Analyses consist of two steps:   |
UltraCheck1 Standard
For each set of tests, an UltraCheck1 Standard must also be performed. This is used as a calibration to convert RLU values obtained from ATP assays into actual ATP concentrations:
The results of these protocols are used to calculate valuable control parameters:
Cellular ATP (cATP = tATP–dATP): biomass energy;
Biomass Stress Index (BSI = dATP/tATP): biomass health;
