Preparing to Test

When getting set to perform ATP analyses, there are several preparations that can be made to save time and improve accuracy of the analysis:

Reagents – Remove Luminase from the fridge 1 hour in advance of any analyses to allow them to warm to room temperature. Choose a work area that is relatively stable at 20 C to 25 C (68 F - to 78 F)

• Be certain to allow Luminase to reach ambient temperature prior to use!

• For room temperature (15 to 25 oC) storage, no warming is required.
• For refrigerator (2 to 8 oC) storage, let stand at ambient for at least 1 hour prior to testing.
• For freezer (-10 to -20 oC) storage, let stand at ambient for at least 6 hours prior to testing.

Micropipetting – The most important aspect to ensure accurate results of the proper use of the micropipettor for sample transfer. Micropipettors are a highly accurate means of pipetting and are relatively easy to use.

Although adjustable micropipettors offer greater flexibility, fixed-volume micropipettors may be used to save time by avoiding adjustments.