Specificity
The GeneCount® qPCR Total Bacteria assay is designed to target specific bacterial genes. Each primer is designed to amplify this target and are meticulously selected using the most up-to-date reference databases. They are then screened both computationally and manually to ensure target detection with little off-target interference.
Coverage
During assay design, the genomes of thousands of different organisms are screened until each primer and probe has the right combination of specificity and breadth of target recognition.
Compatibility
Although assays are designed around samples extracted using the GeneCount® qKit Purifiers, and the analysis features of the GeneCount® instruments, they are also designed to be compatible with many standard DNA purification methods and 4+ channel qPCR instruments.
Efficiency
Through careful optimization of assay reagents, reaction parameters, and calculations integrated with the GeneCount® software, assays must demonstrate limits of detection (LOD) and quantification (LOQ) within industry actionable limits, giving you results that matter.
Robust & repeatable
Through extensive screening and analysis with real-world samples extracted from target sample matrices (water, wastewater, fuel, etc.), GeneCount® assays are capable of consistently detecting their target organism even within difficult sample types.
